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Sclerotization

Biochemical process in insects


Biochemical process in insects

Sclerotization is a biochemical process that produces the rigid shell of sclerotin that comprises an insect's chitinous exoskeleton. It is prominent in the thicker, armored parts of insects and arachnids, especially in the biting mouthparts and sclerites of scorpions and beetles.

Molecular mechanism

Sclerotization entails crosslinking of oxygen-reactive derivatives of dopamine. The reaction of the dopamine derivatives toward oxygen is catalyzed by diverse enzymes such as laccase, which convert the catechol groups to quinones. The resulting quinones are susceptible to nucleophilic attack by amines and thiols, which decorate the side-chains of proteins. These reactions gives rise to color (typically brown), loss of solubility, and rigidification that accompany sclerotization.

References

References

  1. (2010). "Insect Cuticular Sclerotization: A Review". Insect Biochemistry and Molecular Biology.
  2. (2001). "Oxidative Conjugation of Catechols with Proteins in Insect Skeletal Systems". Tetrahedron.
  3. (2006). "When Quinones Meet Amino Acids: Chemical, Physical and Biological Consequences". Amino Acids.
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