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Rhizobium leguminosarum exopolysaccharide glucosyl ketal-pyruvate-transferase

Field	Value
Name	Rhizobium leguminosarum exopolysaccharide glucosyl ketal-pyruvate-transferase
EC_number	2.5.1.98

Rhizobium leguminosarum exopolysaccharide glucosyl ketal-pyruvate-transferase (, PssM) is an enzyme with systematic name *phosphoenolpyruvate:(D-GlcA-beta-(1-4)-2-O-Ac-D-GlcA-beta-(1-4)-D-Glc-beta-(1-4)-(3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1-4)-D-Glc-beta-(1-4)-D-Glc-beta-(1-4)-D-Glc-beta-(1-6))-2(or3)-O-Ac-D-Glc-alpha-(1-6))n 4,6-O-(1-carboxyethan-1,1-diyl)transferase *. This enzyme catalyses the following chemical reaction

: phosphoenolpyruvate + [D-GlcA-beta-(1-4)-2-O-Ac-D-GlcA-beta-(1-4)-D-Glc-beta-(1-4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1-4)-D-Glc-beta-(1-4)-D-Glc-beta-(1-4)-D-Glc-beta-(1-6)]-2(or3)-O-Ac-D-Glc-alpha-(1-6)]n \rightleftharpoons [D-GlcA-beta-(1-4)-2-O-Ac-D-GlcA-beta-(1-4)-D-Glc-beta-(1-4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1-3)-4,6-CH3(COO-)C-D-Glc-beta-(1-4)-D-Glc-beta-(1-4)-D-Glc-beta-(1-6)]-2(or3)-O-Ac-D-Glc-alpha-(1-6)]n + phosphate

The enzyme is responsible for pyruvylation of subterminal glucose in the acidic octasaccharide repeating unit of the exopolysaccharide of Rhizobium leguminosarum.

References

(August 2010). "Mutation in the pssM gene encoding ketal pyruvate transferase leads to disruption of Rhizobium leguminosarum bv. viciae-Pisum sativum symbiosis". Journal of Applied Microbiology.

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ec-2.5.1

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