Protein kinase N1

Function

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Interactions

Protein kinase N1 has been shown to interact with:

• AKAP9,
• Actinin, alpha 1,
• CCDC85B,
• NEFL,
• NEUROD2
• Phosphoinositide-dependent kinase-1,
• Phospholipase D1,
• RHOA, and
• Vimentin.
• TRAF1.

References

References

1. (June 1998). "The protein kinase N (PKN) gene PRKCL1/Prkcl1 maps to human chromosome 19p12-p13.1 and mouse chromosome 8 with close linkage to the myodystrophy (myd) mutation". Genomics.
2. "Entrez Gene: PKN1 protein kinase N1".
3. (June 1999). "Characterization of a novel giant scaffolding protein, CG-NAP, that anchors multiple signaling enzymes to centrosome and the golgi apparatus". J. Biol. Chem..
4. (January 2002). "Pathological shear stress stimulates the tyrosine phosphorylation of alpha-actinin associated with the glycoprotein Ib-IX complex". Biochemistry.
5. (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature.
6. (April 1996). "PKN associates and phosphorylates the head-rod domain of neurofilament protein". J. Biol. Chem..
7. (December 1999). "Interaction of PKN with a neuron-specific basic helix-loop-helix transcription factor, NDRF/NeuroD2". Brain Res. Mol. Brain Res..
8. (July 2000). "A 3-phosphoinositide-dependent protein kinase-1 (PDK1) docking site is required for the phosphorylation of protein kinase Czeta (PKCzeta ) and PKC-related kinase 2 by PDK1". J. Biol. Chem..
9. (May 2001). "PKN regulates phospholipase D1 through direct interaction". J. Biol. Chem..
10. (June 2003). "RhoE binds to ROCK I and inhibits downstream signaling". Mol. Cell. Biol..
11. (April 1998). "Analysis of RhoA-binding proteins reveals an interaction domain conserved in heterotrimeric G protein beta subunits and the yeast response regulator protein Skn7". J. Biol. Chem..
12. (January 1998). "Multiple interactions of PRK1 with RhoA. Functional assignment of the Hr1 repeat motif". J. Biol. Chem..
13. (May 1997). "Domain-specific phosphorylation of vimentin and glial fibrillary acidic protein by PKN". Biochem. Biophys. Res. Commun..
14. (May 2008). "Negative regulation of constitutive NF-kappaB and JNK signaling by PKN1-mediated phosphorylation of TRAF1". Genes Cells.