Pollen count

History

In the UK, the public announcement of the pollen count was popularised by Dr. William Frankland, an immunologist. The National Pollen and Aerobiology Research Unit became the world's first pollen forecasting service in 1983. According to a study by Leonard Bielory, M.D. that was presented to the American College of Allergy, Asthma & Immunology in 2012, climate changes are expected to cause pollen counts to more than double by 2040.

Methods

One method for sampling pollen from the air is the Burkard trap, also known as the seven-day volumetric spore trap, which works by facing towards the wind and drawing in air using a pump. The pollen particles drawn in by the pump are then stuck to a silicone grease-coated tape that is attached to a rotating drum. The drum slowly rotates one turn over the course of seven days while collecting particles. The tape is removed after one week, cut into day-length sections, and these sections are mounted on a microscope slide with fuchsine-stained gelatine. The fuchsine selectively stains plant material magenta, making the pollen easy to differentiate from bycatch when analysed under a light microscope. The tape is typically analyzed in a longitudinal transect to account for circadian differences in pollen prevalence. The number of pollen grains in a given volume of air can then be calculated by entering the pollen counts into a mathematical formula that account for the sampling conditions.

An alternative method uses a rotating silicone grease-coated rod, called a rotorod sampler, rather than a Burkard trap.

Machines that use a camera paired with computer software to automatically count and identify pollen samples in the field are being developed and tested. This has the potential to save time, standardise data collection, and provide real-time pollen counts without delay.

Metagenomics may be used to circumvent pollen counting entirely. This method has the advantage of facilitating narrower taxonomic identifications than are typically possible with microscopy, but it is more costly.

References

References

1. Skinner, Anne. (Aug 6, 2016). "What is Pollen?". Master Gardener Newspaper.
2. "The Year 2040: Double the Pollen, Double the Allergy Suffering?".
3. (2002-03-01). "Seasonal variations in the atmospheric Betula pollen count in Gdańsk (southern Baltic coast) in relation to meteorological parameters". Aerobiologia.
4. (2006). "The Air Spora - Springer".
5. (16 September 2012). "Rotorod airborne pollen sampler".
6. Frenz, D. A.. (2000-11-01). "The effect of windspeed on pollen and spore counts collected with the Rotorod Sampler and Burkard spore trap". Annals of Allergy, Asthma & Immunology.
7. (2001-11-01). "A rapid, reproducible method for coating Rotorod Sampler collector rods with silicone grease". Annals of Allergy, Asthma & Immunology.
8. Frenz, D. A.. (1999-11-01). "Comparing pollen and spore counts collected with the Rotorod Sampler and Burkard spore trap". Annals of Allergy, Asthma & Immunology.
9. (1999-09-01). "Pollen recovery in atmospheric samples collected with the Rotorod Sampler over multiple-day periods such as weekends". Annals of Allergy, Asthma & Immunology.
10. (1997-09-01). "A comparison of pollen recovery by three models of the Rotorod sampler". Annals of Allergy, Asthma & Immunology.
11. (1996-03-01). "A calibration program for Rotorod samplers". Annals of Allergy, Asthma & Immunology.
12. (2004-01-27). "Method of making a pre-greased collecting rod assembly for pollen and fungal spore sampling".