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Methylsterol monooxygenase

Class of enzymes

Summary

Class of enzymes

FieldValue
NameMethylsterol monooxygenase
EC_number1.14.13.72
CAS_number37256-80-7

Methylsterol monooxygenase (, methylsterol hydroxylase, 4-methylsterol oxidase, 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol,hydrogen-donor:oxygen oxidoreductase (hydroxylating)) is an enzyme with systematic name 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol,NAD(P)H:oxygen oxidoreductase (hydroxylating). This enzyme catalyses the following chemical reaction

: 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + 3 NAD(P)H + 3 H+ + 3 O2 \rightleftharpoons 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + 3 NAD(P)+ + 4 H2O (overall reaction) :(1a) 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + NAD(P)H + H+ + O2 \rightleftharpoons 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + NAD(P)+ + H2O :(1b) 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + NAD(P)H + H+ + O2 \rightleftharpoons 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + NAD(P)+ + 2 H2O :(1c) 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + NAD(P)H + H+ + O2 \rightleftharpoons 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + NAD(P)+ + H2O

Methylsterol monooxygenase requires cytochrome b5.

References

References

  1. (July 1997). "The role of cytochrome b5 in 4alpha-methyl-oxidation and C5(6) desaturation of plant sterol precursors". Biochemical and Biophysical Research Communications.
  2. (June 1993). "Plant sterol biosynthesis. Identification and characterization of two distinct microsomal oxidative enzymatic systems involved in sterol C4-demethylation". The Journal of Biological Chemistry.
  3. (March 1986). "Affinity chromatography of microsomal enzymes on immobilized detergent-solubilized cytochrome b5". The Journal of Biological Chemistry.
  4. (May 1981). "Total enzymic synthesis of cholesterol from lanosterol. Cytochrome b5-dependence of 4-methyl sterol oxidase". The Journal of Biological Chemistry.
  5. (November 1980). "Mixed function oxidases in sterol metabolism. Source of reducing equivalents". The Journal of Biological Chemistry.
  6. (October 1968). "Investigation of the component reactions of oxidative sterol demethylation. Evidence against participation of cytochrome P-450". The Journal of Biological Chemistry.
  7. (September 1967). "Investigation of the component reactions of oxidative sterol demethylation. Study of the aerobic and anaerobic processes". Biochemistry.
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