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Methyl blue

Chemical compound

Summary

Chemical compound

| NFPA-H = 1 | NFPA-F = 1 | NFPA-R = 0 | NFPA-S = Methyl blue is a chemical compound with the molecular formula C37H27N3Na2O9S3. It is used as a stain in histology, and stains collagen blue in tissue sections. It can be used in some differential staining techniques such as Mallory's trichrome stain and Gömöri trichrome stain, and can be used to mediate electron transfer in microbial fuel cells. Fungal cell walls are also stained by methyl blue.

Methyl blue is also available in mixture with water blue, under name Aniline Blue WS, Aniline blue, China blue, or Soluble blue; and in a solution of phenol, glycerol, and lactic acid under the name Lactophenol cotton blue (LPCB), which is used for microscopic visualization of fungi.

Chemistry

Methyl blue ([[4-[Bis[4-[(sulfophenyl)amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]amino]-benzenesulfonic acid disodium salt) is distinctly different to methylene blue ([7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;chloride) in structure, function and uses, and must not be confused.

Its uses include staining histology samples for collagen, and for fungal structures.

References

References

  1. R. W. Sabnis. (2010). "Handbook of Biological Dyes and Stains: Synthesis and Industrial Applications". John Wiley & Sons.
  2. "Methyl Blue".
  3. "Methyl blue sodium salt, Histology stain for collagen (CAS 28983-56-4) (Ab146271)".
  4. "Archived copy".
Wikipedia Source

This article was imported from Wikipedia and is available under the Creative Commons Attribution-ShareAlike 4.0 License. Content has been adapted to SurfDoc format. Original contributors can be found on the article history page.

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