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Merthiolate-Iodine-Formaldehyde

Merthiolate-Iodine-Formaldehyde (MIF) is a solution used in biomedical laboratories for concentration of stool samples prior to microscopic investigation for parasites. Concentration of stool is necessary in order to raise sensitivity of microscopy, as in non-concentrated samples the likelihood of finding equivalents of actually present parasites is too low. MIF enables separation of different strata of stool matter, and sedimentation of stool particles that contain parasites (usually parasite eggs). After application of MIF, usually centrifugation is used for acceleration of sedimentation.

The term Merthiolate indicates presence of thiomersal in the solution. Due to the hazardous qualities of thiomersal, it is no longer regularly added to MIF-solutions any more, although the abbreviation MIF is still used for the solution. Thiomersal may nevertheless be added if its preservative qualities are particularly needed, for example if stool samples have to be preserved over longer time periods (for example for teaching samples).

Content

As prepared commercially, 1 L of MIF-solution contains:

  • Ethanolamine 1000 mg
  • Ethylenediamine 280 mg
  • Sodium chloride 8000 mg
  • Disodium-Tetraborate-Decahydrate 2650 mg
  • Distilled water 1000 mL

If Thiomersal is needed for preservation of the sample, add:

  • Thiomersal 1000 mg

Use

For use in laboratory situations, the MIF solution described above is combined with formaldehyde and glycerin in a distilled water dilution.

References

References

  1. Department of Infectious Diseases and Tropical Medicine. ''Dispensary Handbook.'' Ludwig-Maximilians-Universität, Munich
Wikipedia Source

This article was imported from Wikipedia and is available under the Creative Commons Attribution-ShareAlike 4.0 License. Content has been adapted to SurfDoc format. Original contributors can be found on the article history page.

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