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Lys-N

Lys-N is a metalloendopeptidase found in the mushroom Grifola frondosa that cleaves proteins on the amino side of lysine residues. [[Image:N-Lys1.png|thumb|right|Crystal structure of Lys-N with co-ordinated zinc atom.]]

Mass spectrometry

Lys-N is becoming a popular protease used for protein digestion in proteomics experiments. The combination Lys-N proteolytic peptides and mass spectrometry sequencing with ETD creates tandem mass spectra composed mostly of amino terminal peptide fragment ions. This fragmentation pattern facilitates the applicability of these spectra for de novo peptide sequencing.

References

References

  1. (July 1998). "Kinetic characterization of lysine-specific metalloendopeptidases from Grifola frondosa and Pleurotus ostreatus fruiting bodies". Journal of Biochemistry.
  2. [http://www.rcsb.org/pdb/explore/explore.do?structureId=1G12 RCSB Protein Data Bank - RCSB PDB - 1G12 Structure Summary]
  3. (May 2008). "Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase". Nature Methods.
Wikipedia Source

This article was imported from Wikipedia and is available under the Creative Commons Attribution-ShareAlike 4.0 License. Content has been adapted to SurfDoc format. Original contributors can be found on the article history page.

proteomics proteases ec-3.4.24 fungal-proteins
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