GPR97

Signaling

The inositol phosphate (IP3) accumulation, aequorin, and 35S isotope binding assays in overexpressing HEK293 cells have demonstrated coupling of GPR97 to Gαo protein triggering cyclic adenosine monophosphate (cAMP). GPR97 actives cAMP response element-binding protein (CREB), NF-κB, and small GTPases to regulate cellular functions.

Function

Systemic steroid exposure is a therapy to treat a variety of medical conditions and is associated with epigenetic processes such as DNA methylation that may reflect pharmacological responses and/or side effects. GPR97 was found to be differently methylated at CpG sites in the genome of blood cells from patient under systemic steroid treatment. GPR97 is transcribed in immune cells. Gene-deficient mice revealed that Gpr97 is crucial for maintaining B-cell population via constitutive CREB and NF-κB activities. Human lymphatic endothelial cells (LECs) abundantly express GPR97. Silencing GPR97 in human LECs indicated that GPR97 modulates cytoskeletal rearrangement, cell adhesion and migration through regulating the small GTPase RhoA and cdc42. In vertebrates, GPR97 has an indispensable role in the bone morphogenetic proteins (BMP) signaling pathway in bone formation. A microarray meta-analysis revealed that mouse Gpr97 is a direct transcriptional target of BMP signaling in long bone development.

References

References

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2. (Jan 1979). "Ca2+-binding modulator protein in protozoa and myxomycete". Cell Biology International Reports.
3. "Entrez Gene: GPR97 G protein-coupled receptor 97".
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6. (May 2013). "Sticky signaling--adhesion class G protein-coupled receptors take the stage". Science Signaling.
7. (Mar 2012). "A novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis". The EMBO Journal.
8. (Apr 2012). "Signaling property study of adhesion G-protein-coupled receptors". FEBS Letters.
9. (Dec 2012). "Systemic steroid exposure is associated with differential methylation in chronic obstructive pulmonary disease". American Journal of Respiratory and Critical Care Medicine.
10. (10 October 2013). "Gpr97 is essential for the follicular versus marginal zone B-lymphocyte fate decision". Cell Death & Disease.
11. (Dec 2013). "The orphan adhesion G protein-coupled receptor GPR97 regulates migration of lymphatic endothelial cells via the small GTPases RhoA and Cdc42". The Journal of Biological Chemistry.
12. (May 2014). "Microarray meta-analysis identifies evolutionarily conserved BMP signaling targets in developing long bones". Developmental Biology.