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Glycoside hydrolase family 2

Enzyme group

Enzyme group

FieldValue
SymbolGlyco_hydro_2_N
NameGlycosyl hydrolases family 2, sugar binding domain
imagePDB 1jz3 EBI.jpg
captione. coli (lacz) beta-galactosidase-trapped 2-deoxy-galactosyl enzyme intermediate
PfamPF02837
Pfam_clanCL0202
InterProIPR006104
PROSITEPDOC00531
SCOP1bgl
CAZyGH2

In molecular biology, Glycoside hydrolase family 2 is a family of glycoside hydrolases , which are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of 100 different families. This classification is available on the CAZy web site, and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.

Glycoside hydrolase family 2 comprises enzymes with several known activities: beta-galactosidase (EC 3.2.1.23); beta-mannosidase (EC 3.2.1.25); beta-glucuronidase (EC 3.2.1.31). These enzymes contain a conserved glutamic acid residue which has been shown, in Escherichia coli lacZ (), to be the general acid/base catalyst in the active site of the enzyme.

The catalytic domain of Beta-galactosidases have a TIM barrel core surrounded several other largely beta domains. The sugar binding domain of these proteins has a jelly-roll fold. These enzymes also include an immunoglobulin-like beta-sandwich domain.

References

References

  1. (July 1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America.
  2. (September 1995). "Structures and mechanisms of glycosyl hydrolases". Structure.
  3. (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal.
  4. "Home".
  5. (January 2014). "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research.
  6. "Glycoside Hydrolase Family 2".
  7. (December 2018). "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes". Glycobiology.
  8. Withers S. "Glycoside Hydrolase Family 2 (GH_2)". CAZypedia - carbohydrate active enzymes.
  9. (June 1992). "Glu-537, not Glu-461, is the nucleophile in the active site of (lac Z) beta-galactosidase from Escherichia coli". The Journal of Biological Chemistry.
  10. (June 1994). "Three-dimensional structure of beta-galactosidase from E. coli". Nature.
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ec-3.2.1 glycoside-hydrolase-families protein-families
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