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Glycoside hydrolase family 15

FieldValue
SymbolGlyco_hydro_15
NameGlycosyl hydrolases family 15
PfamPF00723
Pfam_clanCL0059
SCOP1glm
CAZyGH15

In molecular biology, glycoside hydrolase family 15 is a family of glycoside hydrolases.

Glycoside hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of 100 different families. This classification is available on the CAZy web site, and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.

Glycoside hydrolase family 15 CAZY GH_15 comprises enzymes with several known activities; glucoamylase (); alpha-glucosidase (); glucodextranase ().

Glucoamylase (GA) catalyses the release of D-glucose from the non-reducing ends of starch and other oligo- or poly-saccharides. Studies of fungal GA have indicated 3 closely clustered acidic residues that play a role in the catalytic mechanism. This region is also conserved in a recently sequenced bacterial GA.

The 3D structure of the pseudo-tetrasaccharide acarbose complexed with glucoamylase II(471) from Aspergillus awamori var. X100 has been determined to 2.4A resolution. The protein belongs to the mainly alpha class, and contains 19 helices and 9 strands.

References

References

  1. (July 1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America.
  2. (September 1995). "Structures and mechanisms of glycosyl hydrolases". Structure.
  3. (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal.
  4. "Home".
  5. (January 2014). "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research.
  6. "Glycoside Hydrolase Family 15".
  7. (December 2018). "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes". Glycobiology.
  8. (January 1990). "Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of Asp176, Glu179 and Glu180 in the enzyme from Aspergillus awamori". Protein Engineering.
  9. (July 1992). "Molecular cloning of a glucoamylase gene from a thermophilic Clostridium and kinetics of the cloned enzyme". European Journal of Biochemistry.
  10. (June 1994). "Refined structure for the complex of acarbose with glucoamylase from Aspergillus awamori var. X100 to 2.4-A resolution". The Journal of Biological Chemistry.
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ec-3.2.1 glycoside-hydrolase-families protein-families
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