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Flavonoid 3',5'-hydroxylase


FieldValue
NameFlavonoid 3',5'-hydroxylase
EC_number1.14.14.81
CAS_number94047-23-1

Flavonoid 3',5'-hydroxylase ( was wrongly classified as in the past) is an enzyme with systematic name flavanone,NADPH:oxygen oxidoreductase. This enzyme catalyses the following chemical reaction

: flavanone + 2 NADPH + 2 H+ + 2 O2 \rightleftharpoons 3',5'-dihydroxyflavanone + 2 NADP+ + 2 H2O (overall reaction) :(1a) flavanone + NADPH + H+ + O2 \rightleftharpoons 3'-hydroxyflavanone + NADP+ + H2O :(1b) 3'-hydroxyflavanone + NADPH + H+ + O2 \rightleftharpoons 3',5'-dihydroxyflavanone + NADP+ + H2O

Flavonoid 3',5'-hydroxylase is a heme-thiolate protein (P-450).

References

References

  1. (October 1994). "Characterization of Flavonoid 3[prime],5[prime]-Hydroxylase in Microsomal Membrane Fraction of Petunia hybrida Flowers". Plant Physiology.
  2. (November 1999). "Expression of chimeric P450 genes encoding flavonoid-3', 5'-hydroxylase in transgenic tobacco and petunia plants(1)". FEBS Letters.
  3. (January 1999). "A cytochrome b5 is required for full activity of flavonoid 3', 5'-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors". Proceedings of the National Academy of Sciences of the United States of America.
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