Endoglycosidase H

Structure and activity

Endoglycosidase H is isolated from Streptomyces plicatus or Streptomyces griseus. Its molecular mass is 29 kDa. The primary structure was described by Robbins et al. in 1984.

Endoglycosidase H cleaves the bond in the diacetylchitobiose core of the oligosaccharide between two N-acetylglucosamine (GlcNAc) subunits directly proximal to the asparagine residue, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine.

It deglycosylates mannose glycoproteins, but the extent and rate of the deglycosylation depends to a high degree on the nature of the glycoproteins. The deglycosylation rate can be increased by denaturation of the glycoproteins (e.g., by carboxymethylation, sulfitolysis or by heating in the presence of sodium dodecyl sulfate). The addition of 0.1 M 2-mercaptoethanol highly increases enzyme activity against glycoproteins containing inter- or intra-molecular disulfide bridges, unlike detergents like Triton X-100, n- Octylglucoside, or zwitterionic detergents.

Biochemical applications

Endoglycosidase H is commonly used by cell biologists to monitor posttranslational modification in the Golgi apparatus. Most proteins destined for the cell surface are translated by ribosomes into the rough endoplasmic reticulum (ER) and translocated into the Golgi. Upon entering the ER a molecule containing 14 sugar subunits is linked en bloc to an asparagine in a selective manner by the enzyme oligosaccharyl transferase. It is this oligosaccharide molecule which is modified by a series of enzymes as the protein moves through the different compartments of the Golgi apparatus. Endoglycosidase H is able to cleave each structure of this oligosaccharide as it is processed until the enzyme Golgi α-mannosidase II removes two mannose subunits. Since all later oligosaccharide structures are resistant to cleavage by endoglycosidase H the enzyme is widely used to report the extent of oligosaccharide processing a protein of interest has undergone.

References

Close enzymes

Endoglycosidases F and D, cleave Glc-Nac

PNGase F (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine_amidase)

References

1. (1977). "Endo-β-''N''-acetylglucosaminidase from fig latex". Biochem. Biophys. Res. Commun..
2. (August 1974). "Endo-β-''N''-acetylglucosaminidase acting on carbohydrate moieties of glycoproteins. Purification and properties of the enzyme from ''Diplococcus pneumoniae''". The Journal of Biological Chemistry.
3. (June 1979). "Cytosolic location of an endo-''N''-acetyl-β-D-glucosaminidase activity in rat liver and kidney". The Biochemical Journal.
4. (January 1980). "Demonstration and cytosolic location of an endo-''N''-acetyl-β-D-glucosaminidase activity towards an asialo-''N''-acetyl-lactosaminic-type substrate in rat liver". The Biochemical Journal.
5. (November 1975). "Structural studies of two ovalbumin glycopeptides in relation to the endo-β-''N''-acetylglucosaminidase specificity". The Journal of Biological Chemistry.
6. (February 1974). "The release of intact oligosaccharides from specific glycoproteins by endo-β-''N''-acetylglucosaminidase H". The Journal of Biological Chemistry.
7. (June 1984). "Primary structure of the ''Streptomyces'' enzyme endo-β-''N''-acetylglucosaminidase H". The Journal of Biological Chemistry.
8. [http://www.acciusa.com/pdfs/northstar/100467-1.pdf Endoglycosidase H, North Star]
9. (September 1984). "Optimizing hydrolysis of ''N''-linked high-mannose oligosaccharides by endo-β-''N''-acetylglucosaminidase H". Analytical Biochemistry.
10. (2002). "Molecular Biology of the Cell". Garland.