Diamond knife

Eye surgery

Diamond knives are used in eye surgery, specifically in refractive surgery.

In particular they are the main tool, together with the microscope, for the radial keratotomy invented by Svyatoslav Fyodorov to correct myopia and for the Mini Asymmetric Radial Keratotomy (M.A.R.K.), invented by Marco Abbondanza to correct astigmatism and cure the first and second stages of keratoconus.{{Cite journal

Ultramicrotomy

Metal microtome knives or razor blades are too soft and dull to cut ultrathin sections. In 1950, Latta and Hartmann discovered that the edge of broken glass could be used to cut thin sections of specimens for transmission electron microscopy. Glass knives are used in ultramicrotomy for trimming specimen blocks and cutting thin and ultrathin sections. A skilled operator can cut a small number of ultrathin sections with a glass knife, while the same operator can use a diamond knife to cut thousands of sections on one area of knife.

Humberto Fernández-Morán discovered that a gem-quality diamond, the hardest known material, could be used to fabricate a more durable knife for use in the ultramicrotomy. Diamond knives are both delicate and expensive, usually costing several thousand dollars. Natural gemstones used to produce diamond knives are usually pale yellow, of regular crystal structure and of the greatest possible purity. The grinding process usually reduces the stones to 50% of their original weight.

The diamond blade is then mounted into a soft metal shaft (Wood's metal) and polished to a very sharp edge. The knife's edge is extremely sharp and free of imperfections, which helps produce ultrathin sections of very regular thickness, to get views of specimens at high magnifications with the transmission electron microscope (TEM). The shaft containing the final edge is then mounted in a metal trough, or "boat", and cemented, usually with an epoxy plastic. Diamond knives are also used for specimen preparation in atomic force microscopy.

Types

There are many styles and types of diamond knives. For the most part, they can be made for use dry or wet. Blade angles of diamond knives vary from 35 to 60 degrees. The smaller angled knives are used for finer cutting, but these knives are prone to damage. Wide angle knives have better life expectancy.

References

References

1. (2021). "The Beginnings of Electron Microscopy - Part 1.". Elsevier Science.
2. (August 6, 1958). "Patente Cuchilla Diamante".
3. Dykstra, Michael J.. (1993). "A Manual of Applied Techniques for Biological Electron Microscopy". Plenum.
4. SOPHIA KISHKOVSKY. (2000-06-04). "Svyatoslav Fyodorov, 72, Eye Surgery Pioneer". New York Times.
5. "Mini cheratomia radiale asimmetrica (mini A.R.K.) per la correzione chirurgica del cheratocono in fase iniziale, nell'ipermetropia e nelle miopie lievi".
6. (24 May 2008). "La curva pericolosa della cornea - Medicina - ilGiornale".
7. (2008). "Zur Aesthesiometrie der Hornhaut nach refraktiver Hornhautchirurgie". Klinische Monatsblätter für Augenheilkunde.
8. (1950). "Use of a Glass Edge in Thin Sectioning for Electron Microscopy". Experimental Biology and Medicine.
9. (1999). ["Electron Microscopy: Principles and Techniques for Biologists"](https://books.google.com/books?id=zMkBAPACbEkC}}{{Page needed). Jones & Bartlett Learning.
10. G. Mahon and T. Malis (1995) Ultramicrotomy of Nano-crystalline Materials. Microscopy Research and Technique, 31, 267-274. {{doi. 10.1002/jemt.1070310403
11. S.R. Glanvill (1995) Ultramicrotomy of Semiconductors and Related Materials. Microscopy Research and Technique, 31, 267-274. {{doi. 10.1002/jemt.1070310404
12. (2021). "The Beginnings of Electron Microscopy - Part 1.". Elsevier Science.
13. [https://www.microstartech.com/index/diamondknives/diamondmanual.htm Micro Star Technologies, diamond knives]
14. [https://www.microstartech.com/index/diamondknives/diamondmanual.htm Micro Star Technologies, diamond knives]
15. Xu, J.. (1 December 2003). "Atomic force microscopy and nanoindentation characterization of human lamellar bone prepared by microtome sectioning and mechanical polishing technique". Journal of Biomedical Materials Research.