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Deoxyhypusine synthase

FieldValue
NameDeoxyhypusine synthase
EC_number2.5.1.46
CAS_number127069-31-2

Deoxyhypusine synthase (, spermidine:eIF5A-lysine 4-aminobutyltransferase (propane-1,3-diamine-forming)) is an enzyme with systematic name (eIF5A-precursor)-lysine:spermidine 4-aminobutyltransferase (propane-1,3-diamine-forming). This enzyme catalyses the following chemical reaction

: [eIF5A-precursor]-lysine + spermidine \rightleftharpoons [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine (overall reaction) : (1a) spermidine + NAD+ \rightleftharpoons dehydrospermidine + NADH : (1b) dehydrospermidine + [enzyme]-lysine \rightleftharpoons N-(4-aminobutylidene)-[enzyme]-lysine + propane-1,3-diamine : (1c) N-(4-aminobutylidene)-[enzyme]-lysine + [eIF5A-precursor]-lysine \rightleftharpoons N-(4-aminobutylidene)-[eIF5A-precursor]-lysine + [enzyme]-lysine : (1d) N-(4-aminobutylidene)-[eIF5A-precursor]-lysine + NADH + H+ \rightleftharpoons [eIF5A-precursor]-deoxyhypusine + NAD+

The eukaryotic initiation factor eIF5A contains a hypusine residue that is essential for activity.

References

References

  1. (April 1990). "Pro-alpha 1(XI) collagen. Structure of the amino-terminal propeptide and expression of the gene in tumor cell lines". The Journal of Biological Chemistry.
  2. (June 1997). "Enzyme-substrate intermediate formation at lysine 329 of human deoxyhypusine synthase". The Journal of Biological Chemistry.
  3. (1997). "Biochemistry and function of hypusine formation on eukaryotic initiation factor 5A". Biological Signals.
  4. (November 1999). "Deoxyhypusine synthase from tobacco. cDNA isolation, characterization, and bacterial expression of an enzyme with extended substrate specificity". The Journal of Biological Chemistry.
  5. (December 1999). "Homospermidine synthase, the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis, evolved from deoxyhypusine synthase". Proceedings of the National Academy of Sciences of the United States of America.
  6. (January 1999). "Identification of lysine350 of yeast deoxyhypusine synthase as the site of enzyme intermediate formation". Yeast.
  7. (March 2000). "Deoxyhypusine synthase generates and uses bound NADH in a transient hydride transfer mechanism". The Journal of Biological Chemistry.
  8. (September 1995). "Cloning and expression of human deoxyhypusine synthase cDNA. Structure-function studies with the recombinant enzyme and mutant proteins". The Journal of Biological Chemistry.
  9. (October 1995). "Molecular cloning and functional expression of Neurospora deoxyhypusine synthase cDNA and identification of yeast deoxyhypusine synthase cDNA". The Journal of Biological Chemistry.
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