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COS cells

Cell lines derived from monkey kidney tissue

COS cells
Summary

Cell lines derived from monkey kidney tissue

COS-7 cells, 40X magnification

COS are fibroblast-like cell lines derived from monkey kidney tissue. COS cells are obtained by immortalizing CV-1 cells with a version of the SV40 virus that can produce large T antigen but has a defect in genomic replication. The CV-1 cell line in turn was derived from the kidney of the African green monkey.

The acronym "COS" is derived from the cells being CV-1 (simian) in Origin, and carrying the SV40 genetic material. Three COS lines were created (COS-1, COS-3 and COS-7), of which two are commonly used (COS-1 and COS-7).

Applications

The COS cell lines are often used by biologists when studying the monkey virus SV40. Cells from these lines are also often transfected to produce recombinant proteins for molecular biology, biochemistry, and cell biology experiments.

When an expression construct with an SV40 origin of replication is introduced into COS cells, the vector can be replicated substantially by the large T antigen. These COS cells are genetically modified to produce the T antigen from their own genome.

References

References

  1. (July 1964). "Infection of human and simian tissue cultures with rous sarcoma virus". Proc. Natl. Acad. Sci. U.S.A..
  2. Gluzman Y. (January 1981). "SV40-transformed simian cells support the replication of early SV40 mutants". Cell.
  3. (2016). "COS-7 cell line: Origin and Characteristics".
  4. (November 2002). "Transient expression of proteins using COS cells". Curr Protoc Mol Biol.
  5. (1995). "Heterologous expression of rat liver microsomal glutathione transferase in simian COS cells and Escherichia coli". Biochem. J..
Wikipedia Source

This article was imported from Wikipedia and is available under the Creative Commons Attribution-ShareAlike 4.0 License. Content has been adapted to SurfDoc format. Original contributors can be found on the article history page.

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