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Beta-carotene 3-hydroxylase

Class of enzymes


Class of enzymes

FieldValue
NameBeta-carotene 3-hydroxylase
EC_number1.14.13.129
CAS_number133425-64-6

Beta-carotene 3-hydroxylase (, beta-carotene 3,3'-monooxygenase, CrtZ) is an enzyme with systematic name *beta-carotene,NADH:oxygen 3-oxidoreductase *. This enzyme catalyses the following chemical reaction

: beta-carotene + 2 NADH + 2 H+ + 2 O2 \rightleftharpoons zeaxanthin + 2 NAD+ + 2 H2O (overall reaction) :(1a) beta-carotene + NADH + H+ + O2 \rightleftharpoons beta-cryptoxanthin + NAD+ + H2O :(1b) beta-cryptoxanthin + NADH + H+ + O2 \rightleftharpoons zeaxanthin + NAD+ + H2O

Beta-carotene 3-hydroxylase requires ferredoxin and Fe(II).

References

References

  1. (October 1996). "Cloning and functional analysis of the beta-carotene hydroxylase of Arabidopsis thaliana". The Journal of Biological Chemistry.
  2. (March 1997). "In vitro characterization of astaxanthin biosynthetic enzymes". The Journal of Biological Chemistry.
  3. (March 1998). "Enzymic confirmation of reactions involved in routes to astaxanthin formation, elucidated using a direct substrate in vitro assay". European Journal of Biochemistry.
  4. (April 1998). "Xanthophyll biosynthesis: molecular and functional characterization of carotenoid hydroxylases from pepper fruits (Capsicum annuum L.)". Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism.
  5. (September 1999). "Carotenoid hydroxylase from Haematococcus pluvialis: cDNA sequence, regulation and functional complementation". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression.
  6. (February 2003). "cDNAs for the synthesis of cyclic carotenoids in petals of Gentiana lutea and their regulation during flower development". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression.
  7. (October 2006). "Characterization of bacterial beta-carotene 3,3'-hydroxylases, CrtZ, and P450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in Escherichia coli". Applied Microbiology and Biotechnology.
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