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Baby hamster kidney cell

BHK21 cells cultured on flexible interfacial area of PFC / culture medium system

Baby Hamster Kidney fibroblasts (BHK cells) are an adherent cell line used in molecular biology. The cells were derived in 1961 by I. A. Macpherson and M. G. P. Stoker. Nowadays, subclone 13 is occasionally used, which was originally derived by single-cell isolation from the kidneys of five unsexed, 1-day-old hamsters.

BHK and virus infection

BHK-21 cells are susceptible to human adenovirus D, reovirus 3, and vesicular stomatitis virus (Indiana strain). BHK-21 cells are resistant to poliovirus 2 and Rabbit vesivirus (RaV). The cells are negative for reverse transcriptase, which means that they lack integral retrovirus genomes.

Utilization

The BHK-21 cells are useful for transformations and for stable and temporary transfections.

Recommended growth medium

High-glucose DMEM
FBS fetal bovine serum: 5% (15% when freeze)
GLU glutamine: 1%
PSA regular antibiotics: 1%
Splitting: 10 by trypsin

References

(August 2015). "Robust kinetics of an RNA virus: Transcription rates are set by genome levels". Biotechnology and Bioengineering.
"BHK-21 Cells".
(September 2006). "Model-based design of growth-attenuated viruses". PLOS Computational Biology.
"Microscopy Resource Center {{!}} Olympus Life Science".

Wikipedia Source

This article was imported from Wikipedia and is available under the Creative Commons Attribution-ShareAlike 4.0 License. Content has been adapted to SurfDoc format. Original contributors can be found on the article history page.

rodent-cell-lines virology

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