3-hydroxyacyl-CoA dehydrogenase

In enzymology, a 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) is an enzyme that catalyzes the chemical reaction

(S)-3-hydroxyacyl-CoA + NAD+

    ⇌
  

{\displaystyle \rightleftharpoons }

3-oxoacyl-CoA + NADH + H+

Thus, the two substrates of this enzyme are (S)-3-hydroxyacyl-CoA and NAD+, whereas its 3 products are 3-oxoacyl-CoA, NADH, and H+.

This enzyme belongs to the family of oxidoreductases, to be specific those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.

In humans, the following genes encode proteins with 3-hydroxyacyl-CoA dehydrogenase activity:

• HADH – Hydroxyacyl-Coenzyme A dehydrogenase
• HSD17B10 – 3-Hydroxyacyl-CoA dehydrogenase type-2
• EHHADH – Peroxisomal bifunctional enzyme
• HSD17B4 – Peroxisomal multifunctional enzyme type 2

3-Hydroxyacyl CoA dehydrogenase is classified as an oxidoreductase. It is involved in fatty acid metabolic processes. Specifically it catalyzes the third step of beta oxidation; the oxidation of L-3-hydroxyacyl CoA by NAD+. The reaction converts the hydroxyl group into a keto group.

The end product is 3-ketoacyl CoA.

This enzyme participates in 8 metabolic pathways:

The systematic name of this enzyme class is (S)-3-hydroxyacyl-CoA:NAD+ oxidoreductase. Other names in common use include:

As of 20 January 2010, 22 structures have been solved for this class of enzymes, with PDB accession codes 1F0Y, 1F12, 1F14, 1F17, 1F67, 1GZ6, 1IKT, 1IL0, 1LSJ, 1LSO, 1M75, 1M76, 1S9C, 1WDK, 1WDL, 1WDM, 1ZBQ, 1ZCJ, 2D3T, 2HDH, 3HAD, and 3HDH.

• Acetoacetyl-CoA reductase acting on the enantiomer of the substrate
• 3-hydroxy-2-methylbutyryl-CoA dehydrogenase